Rustler Railroad is the 3rd area that appears in Cactus McCoy and the Curse of Thorns. It is one of three areas that take place in a train. Other train areas in Cactus McCoy and the Curse of Thorns are Midnight Express and Deadridge Railway.
- Luggage Bandit: Rummage through all the suitcases.
- High-Speed Train: Beat the level in 1:30.
- Train Inspector: Explore all of the train cars.
- Cherrybomber: Kill 20 Enemigos using Cherry Bombs.
- Combo Train: Get a x50 Combo while fighting.